| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Development of four EST-SSR multiplex PCRs in the Pacific oyster (Crassostrea gigas) and their validation in parentage assignment
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| Autores: |
Li, Ronghua
Li, Qi
Cornette, Florence
Degremont, Lionel
Lapegue, Sylvie
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| Data: |
2010-12
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| Ano: |
2010
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| Palavras-chave: |
Pacific oyster
Crassostrea gigas
Microsatellite
EST
Multiplex PCR
Parentage assignment
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| Resumo: |
We report four highly informative multiplex PCRs developed from twelve previously described ESTSSRs in Crassostrea gigas. We evaluated and validated these multiplex PCRs in twelve full-sib families. The average allelic richness and the polymorphism information content (PIC) were 11.1 and 0.811 respectively. The combined power of exclusion was greater than 99.99% using all four multiplex assays. A hundred and forty three tests of segregation ratios revealed 11 significant departures from expected Mendelian ratios. The frequency of null alleles was estimated as 4.9% of all the alleles segregating based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 97% of all offspring were unambiguously allocated to a pair of parents based on two multiplex PCRs with only a 4% error rate, and 100% of the offspring were correctly allocated to their parents when three multiplex PCRs were used.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00013/12419/9999.pdf
DOI:10.1016/j.aquaculture.2010.09.037
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| Editor: |
Elsevier Science Bv
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| Relação: |
http://archimer.ifremer.fr/doc/00013/12419/
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| Formato: |
application/pdf
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| Fonte: |
Aquaculture (0044-8486) (Elsevier Science Bv), 2010-12 , Vol. 310 , N. 1-2 , P. 234-239
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| Direitos: |
2010 Elsevier B.V. All rights reserved.
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